Dare to Discover

A precision DNA & RNA synthesis platform to empower cell & gene therapy pioneers to explore the most complex scientific questions with unparalleled depth and clarity.

Our Foundry

DNA platform

Streamlined development solutions for plasmids and libraries for viral vector and mRNA therapeutics.

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mRNA platform

Industry leading mRNA development and manufacturing solutions.

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Industry leading discovery and optimisation with precision DNA synthesis 

Precise variant control and representation of codons for protein expressing sequences. Specify an exact combination of codons, a precise percentage of desired amino acids and reduce frameshift mutations for the best mutant integrity.

Or introduce singular mutations in a user-defined region or truncate a sequence to characterize and optimize the desired protein features and function.

A powerful tool for determining and optimizing biomolecule function from a parent sequence.

Create a library of randomized, partially randomized or specifically designated mutants with multiple, different combinations of genetic elements. Precisely create codon or DNA part variants in combination to efficiently explore the vast design space. 

Design DNA for functionality while we handle the construction. With DNA construct synthesis up to 50kbp and none of the complexity limits of traditional manufacture methods, you concentrate on design while we bring your creations to life. 

CDS libraries

Precise variant control and representation of codons for protein expressing sequences. Specify an exact combination of codons, a precise percentage of desired amino acids (as low as 5%) and reduce frameshift mutations for the best mutant integrity. Or introduce singular/scanning site-directed mutagenesis in a user-defined region or truncate a sequence to characterize and optimize the desired protein features and function.

Combinatorial Libraries

A powerful tool for determining and optimizing biomolecule function from a parent sequence.
Create a library of completely randomized (NNN) or partially randomized (NNK/NNS) mutants with multiple, different combinations of genetic elements. Precisely create codon or DNA part variants in combination to efficiently explore the vast design space.  

Complex Construct Synthesis

Design DNA for functionality while we handle the construction. With DNA construct synthesis up to 50kbp and none of the complexity limits of traditional manufacture methods, you concentrate on design while we bring your creations to life. 

Complex design as standard

Explore a near limitless sequence space with industry leading standards on GC content (15-85%), long stretches of repeating sequences and homopolymers (150bp),

 

Work with collections

Mix and match de novo synthesis of your target sequences with in vivo verified parts from our collections. Or synthesise, store and reuse your own frequently used parts. That way you can save time and budget on your projects.

 

Precise Codon and Amino Acid representation

Control precisely the codon and amino acid representation in your library to match requirements for your research. Our precision synthesis services lets you balance or bias representation as needed.

Highly Diverse, Highly Complex

Our precision DNA synthesis platform allows you to explore the entire sequence space, without compromise. 

Library diversity upto 10^9 and capabilities to produce highly complex libraries with variants across multiple non-contiguous sequences of interest effectively multiplies your discovery potential.

Specifications

Delivery and Format

We deliver constructs as lyophilised DNA with options from research grade to endotoxin-free purity at milligram scales and linear or circular form.


Mutant libraries can be delivered as mixed pool of variants or as individual subpools.

Cloned Libraries
  • Up to 5mg of plasmid (high copy) 
Linear DNA and RNA libraries
  • 500 ng-1 µg
Destination vectors

Libraries can be delivered into your own custom destination vector or one of our own standard destination vectors suitable for your downstream screening protocols.

Amino Acid and Codon Control

We enable the precise encoding of amino acid variants.

Uniform Amino Acid representation:
For example, we can manage uniform codon usage schemes for example G125 to 25% A, 25% V, 25% L, 25% Q),

Biased Amino Acid representation:
We can also provide more complex, biased representation schemes for example G125 to 60% A, 20% V, 20% L.

Codon Optimisation and Representation:
We can optimise coding sequences for your target organism of interest or control precisely the balance of codon usage in your library to support discovery goals.

Degenerate Codon Libraries:
We can also provide the following degenerate libraries:

Degenerate codon   No. of codons No. of amino acids No. of stops Amino acids encoded
NNN 64 20 3 All 20
NNK / NNS 32 20 1 All 20
NDT 12 12 0 RNDCGHILFSYV
DBK 18 12 0 ARCGILMFSTWV
NRT 8 8 0 RNDCGHSY
Sequence Verification

We offer both next-generation sequencing (NGS) and Sanger sequencing for construct sequence verification and to verify library coverage and diversity.

  • For variants we guarantee 100% sequence verification through sanger sequencing
  • Internal quality standards ensure over 90% coverage for NGS sequencing of libraries
Documentation

Upon delivery of your libraries we will provide:

  • Certificate of Analysis
  • Batch documentation
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In Vitro RNA transcription: Customisation and control for the fourth modality

 Our foundry capabilities allow for in vitro RNA transcription from any DNA template to eliminate traditional constraints faced in research.

High fidelity, purity, and yield allows us to cater to a wide range of research applications. Whether you're delving into expression studies, protein translation or other RNA centric research questions.

Explore and optimise regulatory sequences with in vitro verified DNA parts and targeted sequence variants of 3' and 5' untranslated regions.

RNA transcription

 Our foundry capabilities allow for in vitro RNA transcription from any DNA template to eliminate traditional constraints faced in research.

High fidelity, purity, and yield allows us to cater to a wide range of research applications. Whether you're delving into expression studies, protein translation or other RNA centric research questions.

 

UTR Libraries

Explore and optimise regulatory sequences with in vitro verified DNA parts and targeted sequence variants of 3' and 5' untranslated regions.

Long, complex templates

Explore a near limitless sequence space with industry leading standards on GC content (15-85%), long stretches of repeating sequences and homopolymers (150bp) on CDS up to 10kbp.

Modular Assembly protocols

Elevate your mRNA research with Officinae Bio’s cutting-edge Modular Assembly Protocols by combining in vivo verified part sequences and your own frequently used collections with de novo synthesis.

Explore variants of your transcript with collections of verified promoters and UTRs. Or quickly reuse parts from your own collection that you synthesise and store with us for more effective turnaround.

Precise control over Poly A tails

Our unique Modular Assembly Protocols allow for sequence- and length-perfect polyA tails for DNA templates, giving you unprecedented control over mRNA in vivo stability and translation efficiency.
 
Choose from our flexible length options of Sanger-verified 40, 80, or 120bp to tailor your project to perfection.
 
 
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Providing customisation, speed and cost-efficiency for advanced therapeutic research

Case Study: Combinatorial pathway optimization

We built a combinatorial library for metabolic pathway optimisation. This library explored various elements such as enhancers, repressors, promoters, UTRs, binding sites, localization signals, genes, terminators, and barcodes to fine-tune and maximize the production of the target product.

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Case Study: Mastering sophisticated randomization schemes

We delivered a fully customized library comprising 18.1 million variants. These variants were generated by incorporating user-defined codon usage patterns, which are distributed across multiple non-contiguous positions.

18.1 million variants.
25 days delivery.

Combinatorial Libraries to fine tune mRNA expression

Leverage our sequential approach to optimising mRNA expression for a cost-effective approach to your mRNA construct designs.

 

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We build the tools needed for nucleic acid manufacture so you can focus on creating life changing therapeutics.

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At Officinae Bio, we're developing a platform to support the future of biologically designed therapeutics. We don't ignore biology's complexity, we embrace it. By developing breakthroughs in AI-enabled methodologies for DNA manufacture, biological optimisation and synthetic biology, we help you develop the next generation in advanced therapeutics.

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